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You are here: Home / CETAF Members / Countries / Germany / Botanic Garden and Botanical Museum Berlin / DE-BGBM Passport Facilities

DE-BGBM Passport Facilities

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Facilities
  • Service Area Laboratories: One Laboratory - Two Locations.
  • Spread over two locations, the laboratory facilities cover approximately 450 m2. Institutionally, the Service Area Laboratories is affiliated on one side to the Botanic Garden and Botanical Museum, Berlin, and on the other side to the Borsch Working Group at the Institute of Biology, Systematic Botany and Geography of Plants. Both institutions are part of the Freie Universität Berlin.
  • The activities of the laboratory facility located in the Botanical Museum (Königin-Luise-Str. 6-8) mainly focus on genome- and DNA-analyses (including barcoding), micromorphology and ultra-sculpture of botanical surfaces and the cultivation of diatoms. A field emission scanning electron microscope (FE-SEM) is available.
  • The activities of the laboratory facility located in the Botanical Institute (Altensteinstr. 6) mainly focus on DNA/RNA analyses related to population genetics and genome sizing using flow cytometry. In addition, the facility is equipped with a S1-area for cloning purposes.
 
Scientific Instruments
  • SEM (Scanning electron microscopy) - Hitachi SU-8010 FE-SEM
  • Optical microscopy - Multiple Stereo-/ Microscopes incl. Fluorescence, equipped with high res. Cameras (Olympus, Leica, Zeiss)
  • Genetic fingerprinting - Beckmann CEQ 8000 sequencer
  • Polymerase Chain Reaction - in total 8 x 96-well block thermocycler (SensoQuest and PeqLab)
  • DNA Quality testing - Gel-Electrophoresis and UV/VIS (NanoDrop ND1000)
  • Flow cytometry - Attune, applied biosystems
  • Next Generation Sequencing - via BeGenDiv (www.begendiv.de) Roche 454, Roche Junior and Illumina MySeq
  • Furthermore fumehoods, centrifuges, pipettes, pcr-cabinets, laminair flow workbenches, incubators (with light) and numerous -20°C/-35° and 5 x -80°C storage capacity
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